Beclin-1 (Ser15) Antibody；磷酸化Beclin-1抗体-ABBIOTEC

克隆类型 ：

Rabbit Polyclonal Antibody, Phosphospecific Antibody

亚 型 ：

Rabbit Ig

免 疫 原 ：

KLH-conjugated synthetic phosphopeptide encompassing a sequence surrounding Ser15 of human beclin-1.

产品描述 ：

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death. ULK1 protein kinase and the VPS34 lipid kinase are key regulators of autophagy initiation and progression. Activated during low nutrient conditions, ULK1 directly phosphorylates Beclin-1 at multiple sites including Ser15.

适用物种 ：

H, M

应用范围 ：

E, WB, IP

应用说明 ：

Beclin-1 (Ser15) Antibody recognizes endogenous and transfected levels of Beclin-1 only when phosphorylated at Ser15 (Ser14 in mouse). Low endogenous expression levels are at the detection limit of this antibody. E: 1:500-1:2,000; WB: 1:100-1:1,000; IP: 1:100-1:200

别 名 ：

Beclin-1 ; Coiled-coil myosin-like Bcl-2-interacting protein; Protein GT197; BECN1

形 态 ：

Each vial contains 0.1 ml IgG in PBS pH7.4, 0.1% BSA and 20% glycerol. Antibody was purified by immunogen affinity chromatography.

保存条件 ：

Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed 6 months from the date of shipment.

相关文献 ：

[1] Tanji K. et al. 2015 Biochem Biophys Res Commun. PMID# 26299928. [2] Russell R. et al. 2013. Nat Cell Biol. 15(7): 741-50. PMID# 23685627

实验图片 ：

                        
ATG14L stimulates Beclin-1 Ser14 phosphorylation by promoting association with ULK1. Beclin-1 (murine) alone (left panel) or Beclin-1 and ATG14L (right panel) were overexpressed in HEK293 cells. ULK1 was added to each complex and reactions were quenched at the indicated time points. Phosphorylation of murine Beclin-1 was detected at Ser14 by using the Beclin-1 (Ser15) Antibody (Cat. No. 254515). Data kindly provided by Drs. R. Russell and K-L. Guan at University of California San Diego, La Jolla, CA (USA).

Beclin-1 (Ser15) Antibody (Cat. No. 254515) is used in western blot to detect Beclin-1 phosphorylation levels in brain tissues of mice fed with trehalose (left) or maltose (right). Data from Tanji K. et al. 2015.

 

产品链接 ： http://www.abbiotec.com/antibodies/beclin-1-ser15-antibody