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重组蛋白A抗体
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公司新闻/正文

重组蛋白A,重组耐碱蛋白A,Recombinant Protein A

1030 人阅读发布时间:2017-04-13 14:55

品    名:重组蛋白A (Recombinant Protein A, rat-PA)新闻图片1
货    号:PL030362P
规    格:1 mg,10 mg,100 mg,500 mg
产品形式:冻干粉,冻干前溶液为5 mM PB (4 mM Na2HPO4, 1 mM NaH2PO4, pH 7.4)溶液
分 子 量:31.2 kDa(SDS-PAGE约28 kDa)
等 电 点:5.24
A280nm(1 mg/mL):0.191
来    源:大肠杆菌
纯    度:≥95%
耐    酸:> 0.1 M HCl
耐    碱:> 0.1 M NaOH
耐热:60℃ (1小时)
保存条件:-20℃保存
有 效 期:3 年
相关介绍:
        重组耐碱蛋白A在天然金黄色葡萄球菌蛋白A的基础上对蛋白质结构进行了改造,与天然蛋白A相比,去除与Fab的结合位点,增强重组耐碱蛋白A与Fc结合的专一性,同时提高蛋白稳定性和耐碱性。重组耐碱蛋白A与琼脂糖凝胶键合后可制备用于抗体纯化的亲和填料,可从腹水、血清、细胞培养上清或细胞抽提物中分离和纯化多种哺乳动物不同亚型的抗体或包含抗体Fc片段的基因工程重组蛋白。重组耐碱蛋白A的C端加入了一个半胱氨酸以增强其同凝胶的定向共价键合。
Recombinant Protein A,重组蛋白A
 
Catalog Number: PL030362P
Quantity size : 1mg; 10mg;1g 
Resources: Escherichia coli (E. coli)
Purity: ≥95%
 
Background: Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins preferentially through interaction with the heavy chain. It is conjugated to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine for use in immunology and other biological research without affecting the antibody binding site. 
It is also immobilized onto solid supports such as agarose beads for total IgG purifying from ascitic fluid, serum, culture supernatants and cell extract or interest protein.
  
Storage: Store at –20 ºC for three years. Avoid repeated freeze/thaw cycles. The lyophilized protein is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. It is recommended that the product is reconstituted with sterile water into a final concentration of 10 mg/ml freshly.
 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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