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深圳市豪地华拓生物科技有限公司

入驻年限:15

  • 联系人:

    丁先生

  • 所在地区:

    广东 深圳市 南山区

  • 业务范围:

    试剂、抗体、细胞库 / 细胞培养、技术服务、ELISA 试剂盒

  • 经营模式:

    经销商 代理商 生产厂商

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A novel biosensor based on competitive SERS immunoassay and magnetic separation for accurate and sensitive detection of chloramphenicol【产品文献】
AbstractThe accurate and sensitive detection of chloramphenicol (CAP) is particularly imperative to public health and safety. Here, we present a novel sensor for residual CAP detection based on competitive surface-enhanced Raman scattering (SERS) immunoassay and magnetic separation. In this nanosensor, functionalized Au nanoparticles (AuNPs) were labeled with the Raman reporter molecule (e.g. 4,4′-dipyridyl). With the addition of free CAP, a competitive immune reaction was initiated between free CAP and above AuNPs for conjugating with CAP antibody-modified magnetic nanoparticles (MNPs). Instead of the solid substrate, the antibody conjugated-magnetic beads were used as supporting materials and separation tools in the present sensor. With the aid of a magnet, the mixture was removed from the supernatant for concentration effects. This caused obvious change of SERS signal intensity obtained from supernatant. The SERS signals were collected from the supernatant directly, which made the SERS measurements more stable, repeatable and reliable. The proposed SERS-based magnetic immunosensor allows us to detect CAP in a fast, selective and sensitive (1.0 pg/mL) manner over a wide concentration range ( 1–1×104 pg/mL). In addition, these results demonstrate that this immunosensor holds great potential for the detection of antibiotics in real aquatic environment, which is crucial to our life.KeywordsChloramphenicol; Surface-enhanced Raman scattering; Competitive; Magnetic separation

722 人阅读发布时间:2016-04-12 17:26

A novel biosensor based on competitive SERS immunoassay and magnetic separation for accurate and sensitive detection of chloramphenicol【产品文献】

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